Afghanistan Chamber of Commerce and Industries (ACCI) on Saturday asked all the Afghan merchants to end their trade relations with Pakistan.
Officials said although they have signed the transit agreement with Pakistan, but challenges for Afghan merchants have been doubled.
ACCI have also asked the Afghan government to retaliate and to ban Pakistan's transit convoys to Central Asia.
Deputy for ACCI, Khan Jan Alokozai, said that Pakistan refuses to respect the transit agreement which was signed between the two countries and has stopped Afghanistan's trade convoys for no acceptable reason.
"We urge the government to retaliate to Pakistan so that Pakistani merchants and private sectors also face some challenges. At least do not use your enemies' products." Mr Alokozai said.
Meanwhile, Afghan Exports Union accused the Afghan government for not paying attention to act upon transit agreement and making facilities to Afghan merchants.
"Today I want to submit the licence, it depends on you, whether you want to convey it to Parliament or Ministers' Council," a representative for Afghan Exports Union, Hajji Asan, said.
ACCI said besides those trade containers that were stopped by Pakistan before signing the transit agreement, now Pakistan has stopped another 5000 containers, and Afghan merchants will face a loss of around 500,000 dollars every day.